CREB; PHOSPHO; RBX-100UL

Application

Anti-CREB Antibody, pSer133 is a Rabbit Polyclonal Antibody for detection of CREB also known as CREB-1, cAMP-response Element-binding Protein-1 & has been validated in WB.

Immunoblotting: 1:500-1:1,000

Optimal working dilutions must be determined by the end user.

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryTranscription FactorsRNA Binding Protein (RBP)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Epitope: pSer133

Phosphopeptide conjugated to KLH.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Rabbit serum. Liquid. The antibody has been preabsorbed with unphosphorylated peptide. Contains 0.01% sodium azide.

Specificity

Recognizes phosphorylated Serine133 in cAMP response element binding protein (CREB). By immunoblotting the antibody shows a 5-10 fold increase in staining of a 43 kDa protein after treatment of cells with agents that increase intracellular cAMP. The antibody also recognizes phosphorylated ATF-1 due to a common epitope. The antibody has been preabsorbed with unphosphorylated peptide and will prove to be useful for the investigation of CREB phosphorylation.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze-thaw cycles.